People and Oceans: Managing Marine Areas for Human Well-Being

This booklet demonstrates an awakening within the conservation community that the human relationship with coastal and ocean environments must be evaluated in cultural, social, and economic -- as well as ecological -- dimensions. The major insights from this booklet include:People depend on oceans for food security, recreational opportunities, shoreline protection, climate regulation, and other ecosystem services.Marine resources have tremendous economic value that far exceeds current investments in marine governance, and visitors often are willing to pay far more than existing user fees.MMAs improve human well-being by diversifying livelihoods, enhancing incomes, and improving environmental awareness. They also pose challenges, including loss of access to fishing grounds, inequitable distribution of benefits, dependence on project assistance, and unmet expectations.MMAs are influenced by socioeconomic and governance conditions, including benefits exceeding costs, shared benefits, improved livelihood options, strong community participation, accountable management style, supportive local government, enabling legislation, enforced rules, empowerment and capacity building, strong persistent leadership, and involved external agents.Effective MMAs require strong enforcement, including both soft measures (i.e., education, partnerships) and hard measures (i.e., detection, interception, prosecution, and sanctions).Approaches such as buyouts, conservation agreements, and alternative livelihoods provide positive incentives for altering human behavior

Looking Further and Deeper into Environmental Protection, Regulation and Policy Using Environmental DNA (eDNA)

DNA sequencing technologies are transforming how environments are monitored. In this article, we pose the question: is environmental DNA (eDNA) the tool that Aotearoa New Zealand needs, but does not yet realise it does? The step change with eDNA is that genetic ‘breadcrumbs’ left behind in the environment can identify every living thing, from microbes to mammals, thus providing a more nuanced and holistic lens on ecosystems. Using eDNA, we can explore the biological networks that underpin healthy environments. Here we explore whether changes in policy setting, guidance, or pathways for uptake of eDNA are needed. Can eDNA help us make better decisions, inform policy and protections, track restoration, and act as a deterrent to reduce environmental harm

Gallery Characteristics and Life History of the Ambrosia Beetle Trypodendron betulae (Coleoptera: Curculionidae: Scolytinae) in Birch

Trypodendron betulae Swaine distributed attack entrance holes uniformly over the surface of standing stressed sub-canopy birch trees. Male and female pairs constructed galleries consisting of an entrance tunnel about 20 mm in length and then primary and secondary lateral tunnels averaging between 16 and 23 mm in length into the sapwood. Egg niches were constructed in the lateral tunnels after the symbiotic fungus was established in the galleries. Larvae enlarged the niches into cradles. Pupae and eventually teneral adults developed in the cradles. The sex ratio of resulting progeny adults was approximately one to one, and they emerged from galleries in September to overwinter in the litter

Marine Managed Areas: What, Why, and Where

This paper, which focuses on ocean and coastal areas, explores the challenge of public participation by discussing the role of communities in IM. It draws on a decade of collaboration between academics and community partners to outline the community perspective on both the limiting factors and the opportunities, and a state-of-the-art survey of community involvement in IM, parti-cularly in the Canadian Maritimes. The paper highlights the importance of linking communities and governments, and the need to overcome the growing disconnect between the two. It also illustrates the varied experiences of local coastal communities with IM through three concrete examples. These practical examples lead to two specific out-puts: a set of fundamental IM values and attributes from a community perspective, and a four-step process for facilitating and enabling community-focused IM.The conclusion summarizes key outcomes in terms of inclusivity and active involvement of communities

Inferring Kangaroo Phylogeny from Incongruent Nuclear and Mitochondrial Genes

The marsupial genus Macropus includes three subgenera, the familiar large grazing kangaroos and wallaroos of M. (Macropus) and M. (Osphranter), as well as the smaller mixed grazing/browsing wallabies of M. (Notamacropus). A recent study of five concatenated nuclear genes recommended subsuming the predominantly browsing Wallabia bicolor (swamp wallaby) into Macropus. To further examine this proposal we sequenced partial mitochondrial genomes for kangaroos and wallabies. These sequences strongly favour the morphological placement of W. bicolor as sister to Macropus, although place M. irma (black-gloved wallaby) within M. (Osphranter) rather than as expected, with M. (Notamacropus). Species tree estimation from separately analysed mitochondrial and nuclear genes favours retaining Macropus and Wallabia as separate genera. A simulation study finds that incomplete lineage sorting among nuclear genes is a plausible explanation for incongruence with the mitochondrial placement of W. bicolor, while mitochondrial introgression from a wallaroo into M. irma is the deepest such event identified in marsupials. Similar such coalescent simulations for interpreting gene tree conflicts will increase in both relevance and statistical power as species-level phylogenetics enters the genomic age. Ecological considerations in turn, hint at a role for selection in accelerating the fixation of introgressed or incompletely sorted loci. More generally the inclusion of the mitochondrial sequences substantially enhanced phylogenetic resolution. However, we caution that the evolutionary dynamics that enhance mitochondria as speciation indicators in the presence of incomplete lineage sorting may also render them especially susceptible to introgression.This work has been supported by Australian Research Council grants to MJP (DP07745015) and MB (FT0991741). The website for the funder is www.arc.gov.au. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

From Benchtop to Desktop: Important Considerations when Designing Amplicon Sequencing Workflows

Amplicon sequencing has been the method of choice in many high-throughput DNA sequencing (HTS) applications. To date there has been a heavy focus on the means by which to analyse the burgeoning amount of data afforded by HTS. In contrast, there has been a distinct lack of attention paid to considerations surrounding the importance of sample preparation and the fidelity of library generation. No amount of high-end bioinformatics can compensate for poorly prepared samples and it is therefore imperative that careful attention is given to sample preparation and library generation within workflows, especially those involving multiple PCR steps. This paper redresses this imbalance by focusing on aspects pertaining to the benchtop within typical amplicon workflows: sample screening, the target region, and library generation. Empirical data is provided to illustrate the scope of the problem. Lastly, the impact of various data analysis parameters is also investigated in the context of how the data was initially generated. It is hoped this paper may serve to highlight the importance of pre-analysis workflows in achieving meaningful, future-proof data that can be analysed appropriately. As amplicon sequencing gains traction in a variety of diagnostic applications from forensics to environmental DNA (eDNA) it is paramount workflows and analytics are both fit for purpose

The impact of in-cylinder charge motion on lean limit extension and in-pre-chamber mixture preparation in a homogeneous ultra-lean engine

The perpetual desire to conserve fuel is driving strong demand for increased efficiency in spark ignited (SI) engines. A method being increasingly explored to accomplish this goal is lean combustion. Homogeneous ultra-lean combustion with λ > 1.6 has demonstrated the ability to both increase thermal efficiency and significantly reduce engine-out nitrogen oxides (NOx) emissions due to the colder cylinder temperatures innate to combustion with high levels of dilution. The major limitation in developing lean and ultra-lean combustion systems is the less favorable ignition quality of the mixture. This has necessitated the development of higher energy ignition sources. A pre-chamber combustor application known as jet ignition is one such technology, having been researched extensively. Differing types and magnitudes of charge motion are incorporated in SI engines to aid with mixture preparation. The influence of charge motion over lean SI combustion however is less well understood. Additionally, charge motion introduced in the main combustion chamber has the potential to translate to the pre-chamber, thereby affecting pre-chamber mixing and combustion. The effect of charge motion on mixing and combustion comprehensively throughout the engine cycle is unknown and has not been investigated. This study seeks to evaluate the impact of charge motion on mixture preparation and combustion processes in a jet ignition engine. Experimental engine testing is undertaken to quantify the impact of differing levels and types of induced charge motion on pre-chamber and main chamber combustion. An analysis of high speed pressure data from the pre-chamber provides insight into how charge motion affects pre-chamber combustion stability, and how instabilities cascade to the main chamber combustion event. A set of simulations, matched to experimental engine results, is used to develop an understanding of charge motion influence over the complexities of in-pre-chamber phenomena that are not easily observed experimentally. From the synthesis of these data sets, a clear understanding of the role that charge motion plays in homogeneous highly dilute jet ignition engines emerges. This study quantifies the impact that charge motion has on lean limit extension and engine efficiency, identifies optimal charge motion type, and provides a roadmap for engine system optimization

Landscape ideology in the Greater Golden Horseshoe Greenbelt Plan: Negotiating material landscapes and abstract ideals in the city\u27s countryside

We analyze the role of landscape ideology in the recent Ontario Greater Golden Horseshoe (GGH) Greenbelt Plan. Focusing on the “Protected Countryside,” the major land-use designation in the Plan that structures the Greenbelt framework, we explore tensions between abstract ideals of countryside used by policy makers to elicit support for the Plan and people\u27s lived experience of material landscapes of the peri-urban fringe. Approaching “countryside” from the combined perspectives of landscape studies and political ecology, we show how the abstract ideals used to build support for the protection of countryside in the high-level political arena are in tension with existing material landscapes as people experience them. When implementing the Greenbelt Plan, the abstract ideals have to be applied at the landscape level through negotiation with municipalities, property owners, and other interests. In addition to drawing upon more conventionally legitimate explanations for landscape protection based on environmental science and land-use planning principles, the designation of Protected Countryside and the strategies used to implement the Protected Countryside designation at the local level suggest a tentative commitment to recognizing landscape values and collaborative environmental management processes in policy-making. As with any such normative land-use plan, the success of the Greenbelt Plan hinges on the long-term agreement between planning agencies and diverse publics. We demonstrate the usefulness of approaching environmental management challenges at the urban–rural interface by bringing the perspectives of landscape studies and political ecology into implementation processes for land-use management strategies like the Greenbelt. We argue that public participants deserve legitimate collaborative roles in negotiating just and desirable land uses based on their experiences, and provide observations on ways to bring contested goals and tools for achieving them into reflexive negotiations about how landscapes are and should be produced